Speakers - CIRWC 2024

Abstract

Background: Growing evidence indicated that circRNAs played significant roles in the progression of human cancer. Nevertheless, the molecular mechanism and effects of circ_00923304 in GC are still unclear.

Methods: First, qRT-PCR was used to evaluate the levels of circ_00923304/miR-761/ARVCF from GC tissues, GC cells, normal tissues, and gastric epithelial cells. Then, the GC cells were transfected to analyze the proliferation, migration, and invasion of GC cells by MTT, colony formation, and transwell assays. Next, the expressions of miR-761 and ARVCF in GC tissues and cells were examined by qRT-PCR following transfection. The dual-luciferase reporter and RNA pull-down assays explored the target interaction of circ_00923304-miR-761 and ARVCF. In the end, the growth and viability of GC cells were detected by MTT, colony formation, and transwell assays, respectively, following the transfection of GC cells.

Results: In this research, circ_00923304 expressions are significantly up-regulated in GC tissues and cells compared with normal and gastric epithelial cells. We discovered that the knockdown of circ_00923304 remarkably inhibits GC cell proliferation, migration, and invasion. Besides, by predicting binding sites between circ_00923304, miR-761, and ARVCF, we discovered miR-761 is a target of circ_00923304 while ARVCF is a target gene of miR-761. Finally, the results demonstrate that the overexpression of miR-761 and the silence of ARVCF could inhibit the effects of circ_00923304 on proliferation, migration, and invasion in GC cells.

Conclusion: circ_00923304 accelerated the growth and migration of GC cells by regulating the miR-761/ARVCF axis.

Keywords: gastric cancer, circ_00923304, miR-761, ARVCF,, HGC-27